Figure 4.

miR-615 inhibits NF-κB activation. Luciferase activity in IECs and MARC-145 cells. Cells were co-transfected using a dual-luciferase reporter system (pNiFty-luc and pRL-TK vectors, the activities of which indicate NF-κB-promoter activation) and miR-615 mimics or inhibitors for 24 h, and stimulated with poly (I:C) for 24 h during PEDV infection at a multiplicity of infection (MOI) of 1. Luciferase reporter activity of the miR-615 mimics in IECs (A) and MARC-145 cells (C). Luciferase reporter activity of the miR-615 inhibitors in IECs (B) and MARC-145 cells (D). The data are representative of three independent experiments (mean ± SD). ***p < 0.001.