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. 2022 Dec 1;13:1071394. doi: 10.3389/fmicb.2022.1071394

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Copyright © 2022 Zheng, Li, Zhu, Wang, Yin, Zhang, Feng, Yin, Huang and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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miR-615 inhibits the nuclear translocation and downregulates the phosphorylation of p65. (A,C) Immunofluorescence analysis of the nuclear translocation of p65 protein after transfection of miR-615 mimics during PEDV infection in IECs and MARC-145 cells. IECs and MARC-145 cells were transfected with miR-615. Poly (I:C) stimulation was added for 24 h during PEDV infection. Cells were fixed and stained with a rabbit anti-phosphorylated (p)-p65 and MyD88 monoclonal antibodies. Scale bar = 15 μm. (B,D) Western blot analysis for detection of the (p)-p65 and MyD88 proteins.