TABLE 1.
Reports of GPCRs tested for RAMP interaction, followed by summary statistics GPCRs are grouped by family and sorted alphabetically within each family. Key references are provided.
| GPCR | Abbreviation | Uniprot | Family | RAMP |
|---|---|---|---|---|
| Adhesion G-protein coupled receptor F5 | ADGRF5 | Q8IZF4 | Adhesion | RAMP3 (Lorenzen et al., 2019) |
| Cadherin EGF LAG seven-pass G-type receptor 2 | CELSR2 | Q9HCU4 | Adhesion | None (only RAMP2 tested) (Barbash et al., 2019) |
| Frizzled family receptor 1 | FZD1 | Q9UP38 | Frizzled | None (only RAMP2 tested) (Barbash et al., 2019) |
| Calcium-sensing receptor | CaSR | P41180 | Glutamate | RAMP1 and 3 (Bouschet et al., 2008; Bouschet et al., 2005; Desai et al., 2014) |
| Pituitary adenylate-cyclase activating polypeptide type 1 | ADYCAP1R1 | P41586 | Secretin | RAMP1, 2, and 3 (Lorenzen et al., 2019) RAMP2 and 3 (M. Harris et al., preprint, DOI: https://doi.org/10.1101/2021.04.08.436756) |
| Calcitonin receptor-like receptor | CALCRL | Q16602 | Secretin | RAMP1, 2 and 3 (McLatchie et al., 1998) and many others (including solved structures) |
| Calcitonin receptor | CALCR | P30988 | Secretin | RAMP1, 2 and 3 (Armour et al., 1999; Christopoulos et al., 1999) and many others |
| Corticotropin-releasing hormone receptor 1 | CRHR1 | P34998 | Secretin | None (Tasma et al., 2020) RAMP2 (Wootten et al., 2013; Wootten et al., 2013;Bailey et al., 2019) RAMP3 (Lorenzen et al., 2019) RAMP2,3 (M. Harris et al., preprint) |
| Corticotropin-releasing hormone receptor 2 | CRHR2 | Q13324 | Secretin | None (Lorenzen et al., 2019; Tasma et al., 2020) None (only RAMP2 tested) (Bailey et al., 2019) RAMP2,3 (M. Harris et al., preprint) |
| Glucagon receptor | GCGR | P47871 | Secretin | RAMP2 (Christopoulos et al., 2003; Weston et al., 2015; Cegla et al., 2017; McGlone et al., 2021) RAMP1,3 (Lorenzen et al., 2019) RAMP1,2,3 (Shao et al., 2022; M. Harris et al., preprint) |
| Growth hormone-releasing hormone | GHRHR | Q02643 | Secretin | None (Christopoulos et al., 2003) RAMP2,3 (Lorenzen et al., 2019) RAMP1,2,3 (Shao et al., 2022; M. Harris et al., preprint) RAMP1,2 with splice variant 1 of GHRHR (Shao et al., 2022) |
| Gastric inhibitory polypeptide receptor | GIPR | P48546 | Secretin | RAMP1,2,3 (Lorenzen et al., 2019; Shao et al., 2022; M. Harris et al., preprint) |
| Glucagon-like peptide 1 receptor | GLP1R | P43220 | Secretin | None (Christopoulos et al., 2003; Wootten et al., 2013) RAMP2,3 (Shao et al., 2022) RAMP1,2,3 (Lorenzen et al., 2019) (M. Harris et al., preprint) |
| Glucagon-like peptide 2 receptor | GLP2R | O95838 | Secretin | None (Christopoulos et al., 2003) RAMP1,2,3 (Lorenzen et al., 2019; M. Harris et al., preprint) RAMP3 (Shao et al., 2022) |
| Parathyroid hormone 1 receptor | PTH1R | Q03431 | Secretin | RAMP2 (Christopoulos et al., 2003; Nemec et al., 2022) RAMP1,3 (Lorenzen et al., 2019) RAMP2,3 (M. Harris et al., preprint) RAMP3 (Phelps et al., 2005) |
| Parathyroid hormone 2 receptor | PTH2R | P49190 | Secretin | RAMP3 (Christopoulos et al., 2003) RAMP1,2 (Lorenzen et al., 2019) RAMP1,2,3 (M. Harris et al., preprint) |
| Secretin receptor | SCTR | P47872 | Secretin | RAMP3 (Harikumar et al., 2009) RAMP1,2 (Lorenzen et al., 2019) RAMP1,2,3 (Shao et al., 2022; M. Harris et al., preprint) |
| VIP and PACAP receptor 1 | VIPR1 | P32241 | Secretin | RAMP1,2,3 (Christopoulos et al., 2003) (M. Harris et al., preprint) RAMP2,3 (Lorenzen et al., 2019) |
| VIP and PACAP receptor 2 | VIPR2 | P41587 | Secretin | RAMP1,2,3 (Wootten et al., 2013; (M. Harris et al., preprint) RAMP2,3 (Lorenzen et al., 2019) |
| Atypical chemokine receptor 1 | ACKR1 | Q16570 | Rhodopsin | RAMP1,2,3 (Mackie et al., 2019) |
| Atypical chemokine receptor 2 | ACKR2 | O00590 | Rhodopsin | RAMP1,3 (Mackie et al., 2019) |
| Atypical chemokine receptor 3 | ACKR3 | P25106 | Rhodopsin | RAMP2,3 (Lorenzen et al., 2019) RAMP1,2,3 (Mackie et al., 2019) |
| Atypical chemokine receptor 4 | ACKR4 | Q9NPB9 | Rhodopsin | RAMP2,3 (Mackie et al., 2019) |
| Atypical chemokine receptor 5 | ACKR5 | O00421 | Rhodopsin | None (Mackie et al., 2019) |
| Adenosine A2B receptor | ADORA2B | P29275 | Rhodopsin | RAMP2 (Barbash et al., 2019) |
| Beta 2 adrenergic receptor | B2ADR | P07550 | Rhodopsin | None (Mackie et al., 2019; Shao et al., 2022; M. Harris et al., preprint) |
| C-C Chemokine receptor type 1 | CCR1 | P32246 | Rhodopsin | RAMP1,2,3 (Mackie et al., 2019) |
| C-C Chemokine receptor type 2 | CCR2 | P41597 | Rhodopsin | RAMP2,3 (Mackie et al., 2019) |
| C-C Chemokine receptor type 3 | CCR3 | P51677 | Rhodopsin | RAMP1,2,3 (Mackie et al., 2019) |
| C-C Chemokine receptor type 4 | CCR4 | P51679 | Rhodopsin | RAMP1,2,3 (Mackie et al., 2019) |
| C-C Chemokine receptor type 5 | CCR5 | P51681 | Rhodopsin | None (Lorenzen et al., 2019) RAMP2,3 (Mackie et al., 2019) |
| C-C Chemokine receptor type 6 | CCR6 | P51684 | Rhodopsin | RAMP1,2,3 (Mackie et al., 2019) |
| C-C Chemokine receptor type 7 | CCR7 | P32248 | Rhodopsin | None (Lorenzen et al., 2019) RAMP3 (Mackie et al., 2019) |
| C-C Chemokine receptor type 8 | CCR8 | P51685 | Rhodopsin | RAMP3 (Mackie et al., 2019) |
| C-C Chemokine receptor type 9 | CCR9 | P51686 | Rhodopsin | RAMP1,3 (Mackie et al., 2019) |
| C-C Chemokine receptor type 10 | CCR10 | P46092 | Rhodopsin | RAMP1,2,3 (Mackie et al., 2019) |
| Chemokine-like receptor 1 | CMKLR1 | Q99788 | Rhodopsin | RAMP2,3 (Mackie et al., 2019) |
| Chemokine C-X3-C receptor 1 | CX3CR1 | P49238 | Rhodopsin | RAMP1,2,3 (Mackie et al., 2019) |
| C-X-C chemokine receptor type 1 | CXCR1 | P25024 | Rhodopsin | RAMP1,2 (Mackie et al., 2019) |
| C-X-C chemokine receptor type 2 | CXCR2 | P25025 | Rhodopsin | RAMP2,3 (Mackie et al., 2019) |
| C-X-C chemokine receptor type 3 | CXCR3 | P49682 | Rhodopsin | None (Lorenzen et al., 2019) RAMP3 (Mackie et al., 2019) |
| C-X-C chemokine receptor type 4 | CXCR4 | P61073 | Rhodopsin | None (Lorenzen et al., 2019) RAMP1,3 (Mackie et al., 2019) |
| C-X-C chemokine receptor type 5 | CXCR5 | P32302 | Rhodopsin | None (Mackie et al., 2019) |
| C-X-C chemokine receptor type 6 | CXCR6 | O00574 | Rhodopsin | RAMP3 (Mackie et al., 2019) |
| Proteinase-activated receptor 2 | F2RL1 | P55085 | Rhodopsin | None (only RAMP2 tested) (Barbash et al., 2019) |
| Proteinase-activated receptor 2 | F2RL3 | Q96RI0 | Rhodopsin | RAMP2 (Barbash et al., 2019) |
| G-protein coupled receptor 4 | GRP4 | P46093 | Rhodopsin | RAMP1,2,3 (Lorenzen et al., 2019) |
| G protein-coupled estrogen receptor 1 | GPR30 | Q99527 | Rhodopsin | RAMP3 (Lenhart et al., 2013) |
| Melatonin-related receptor | GPR50 | Q15385 | Rhodopsin | None (only RAMP2 tested) (Barbash et al., 2019) |
| Probably G protein-coupled receptor 141 | GPR141 | Q7Z602 | Rhodopsin | RAMP2 (Barbash et al., 2019) |
| Probably G protein-coupled receptor 141 | GPR160 | Q9UJ42 | Rhodopsin | None (only RAMP2 tested) (Barbash et al., 2019) |
| G protein-coupled receptor 176 | GPR176 | Q80WT4 | Rhodopsin | None (only RAMP2 tested) (Barbash et al., 2019) |
| G-protein coupled receptor 182 | GRP182 | O15218 | Rhodopsin | RAMP1,2,3 (Lorenzen et al., 2019) |
| Leucine-rich repeat-containing G-protein coupled receptor 4 | LGR4 | Q9BXB1 | Rhodopsin | None (only RAMP2 tested) (Barbash et al., 2019) |
| P2Y purinoceptor 8 | P2RY8 | Q86VZ1 | Rhodopsin | RAMP2 (Barbash et al., 2019) |
| Neurotensin receptor type 1 | NTSR1 | P30989 | Rhodopsin | None (only RAMP2 tested) (Barbash et al., 2019) |
| δ-type opioid receptor | OPRD1 | P41143 | Rhodopsin | RAMP2 (Barbash et al., 2019) |
| Sphingosine 1-phosphate receptor 1 | S1PR1 | P21453 | Rhodopsin | None (only RAMP2 tested) (Barbash et al., 2019) |
| Chemokine XC receptor 1 | XCR1 | P46094 | Rhodopsin | None (Mackie et al., 2019) |
| Summary statistics | RAMP1 | RAMP2 | RAMP3 | ||
|---|---|---|---|---|---|
| No. of GPCR interactors (at least one study) | 28 | 30 | 41 | ||
| Secretin | Rhodopsin | Glutamate | Adhesion | Frizzled | |
| No. of GPCRs that interact with any RAMP(s) (based on at least one report) | 15 | 29 | 1 | 1 | — |
| No. of GPCRs that don’t interact with any RAMP (based on at least one report) | 5 | 8a | — | 1 | 1 |
| No. of GPCRs in the family | 15 | 719 | 22 | 33 | 11 |
Key references provided.
aAnd 7 additional instances where the GPCR does not appear to interact with a RAMP, but only RAMP2 was tested.