TABLE 3.

Published structures with RAMPs Asterisk (*) indicates structure obtained by cryo-electron microscopy. Otherwise, structures obtained by X-ray crystallography. All structures marked Full include the ECD and TM domains of the RAMP, with no or very poor C-terminal density observed. Stabilizing mutations made to the RAMP and/or GPCR are not listed but may be present.

GPCR	RAMP	Full length?	Relevant molecules present	Other molecules present	PDB Ref	Ref
None	RAMP1	ECD only		MSE	2YX8	Kusano et al. (2008)
None	RAMP2	ECD only	Ca+2		2XVT	Not published (Quigley, 2010)
None	RAMP2	ECD only		MSE	3AQE	Kusano et al. (2012)
CALCRL	RAMP1	ECD (both RAMP and GPCR)	Olcegepant	3N6, 3N7, sulfate ion	3N7S	ter Haar et al. (2010)
CALCRL	RAMP1	MBP–RAMP1 ECD–CALCRL ECD fusion	AM variant	Maltose	5V6Y	Booe et al. (2018)
CALCRL	RAMP1	MBP–RAMP1 ECD–CALCRL ECD fusion	AM2	Maltose, sodium ion	6D1U	Roehrkasse et al. (2018)
CALCRL	RAMP1	ECD + TM (no C terminus), CALCRL full length	CGRP, Gs heterotrimer	Nb35	6E3Y*	Liang et al. (2018a)
CALCRL	RAMP1	ECD (both RAMP and GPCR)	Telcagepant	3N6, N7R	3N7R	ter Haar et al. (2010)
CALCRL	RAMP1	ECD (both RAMP and GPCR)	(unliganded)	sulfate ion, MSE	3N7P	ter Haar et al. (2010)
CALCRL	RAMP1	MBP–RAMP1 ECD–CALCRL ECD fusion	CGRP analog	Maltose, magnesium ion	4RWG	Booe et al. (2015)
CALCRL	RAMP1	ECD (both RAMP and GPCR)	Erenumab (Fab)		6UMG	Garces et al. (2020)
CALCRL	RAMP1	MBP–RAMP1 ECD–CALCRL ECD fusion	Olcegepant	SN6, tetraethyl glycol (PG4), maltose, alpha-D-glucopyranose	6ZIS	Bucknell et al. (2020)
CALCRL	RAMP1	MBP–RAMP1 ECD–CALCRL ECD fusion	HTL22562	tetraethyl glycol (PG4), maltose, alpha-D-glucopyranose	6ZHO	Bucknell et al. (2020)
CALCRL	RAMP1	MBP–RAMP1 ECD–CALCRL ECD fusion	HTL0028125 (macrocycle)	Unknown (PDB entry on hold)	7P0F	Cansfield et al. (2022)
CALCRL	RAMP1	MBP–RAMP1 ECD–CALCRL ECD fusion	Macrocycle compound 13	Unknown (PDB entry on hold)	7P0I	Cansfield et al. (2022)
CALCRL	RAMP1	ECD + TM (no C terminus), CALCRL full length		Detergent micelle	7KNT*	Josephs et al. (2021)
CALCRL	RAMP1	ECD + TM (no C terminus), CALCRL full length	CGRP	Detergent micelle	7KNU*	Josephs et al. (2021)
CALCRL	RAMP2	ECD only		MSE	3AQF	Kusano et al. (2012)
CALCRL	RAMP2	MPB-RAMP2 ECD–CALCRL ECD fusion	AM	Maltose, 1,2-ethanediol	4RWF	Booe et al. (2015)
CALCRL	RAMP2	MPB–RAMP2 ECD–CALCRL ECD fusion	high-affinity AM (37-52) S45R/K46L/S48G/Q50W	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, maltose, formic acid, amino group	6V2E	Booe et al. (2020)
CALCRL	RAMP2	Full	AM, Gs heterotrimer	Nb35	6UUN*	Liang et al. (2020a)
CALCRL	RAMP3	Full	AM2, Gs heterotrimer	Nb35	6UVA*	Liang et al. (2020a)
CALCRL	RAMP3	Full	AM, Gs heterotrimer	Nb35	6UUS*	Liang et al. (2020a)
CALCR	RAMP1	Full	Rat amylin, Gs heterotrimer	Nb35, P42, phosphatidylethanolamine, cholesterol hemisuccinate, palmitic acid, 2-acetamido-2-deoxy-beta-D-glucopyranose	7TYF*	Cao et al. (2022)
CALCR	RAMP1	Full	Salmon CT, Gs heterotrimer	Nb35, cholesterol hemisuccinate, palmitic acid, 2-acetamido-2-deoxy-beta-D-glucopyranose	7TYW*	Cao et al. (2022)
CALCR	RAMP2	Full	Rat amylin, Gs heterotrimer	Nb35, cholesterol hemisuccinate, palmitic acid, 2-acetamido-2-deoxy-beta-D-glucopyranose	7TYX*	Cao et al. (2022)
CALCR	RAMP2	Full	Salmon CT, Gs heterotrimer	Nb35, cholesterol hemisuccinate, palmitic acid, 2-acetamido-2-deoxy-beta-D-glucopyranose	7TYY*	Cao et al. (2022)
CALCR	RAMP2	Full	CT, Gs heterotrimer	Nb35	7TYH*	Cao et al. (2022)
CALCR	RAMP3	Full	Rat amylin, Gs heterotrimer	Nb35, P42, cholesterol hemisuccinate, palmitic acid, 2-acetamido-2-deoxy-beta-D-glucopyranose	7TZF*	Cao et al. (2022)

MBP, maltose binding protein; MSE, selenomethionine, an L-peptide linking molecule; Nb35, Nanobody 35.