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I340T does not form a TMC with C3b and FH CCP1-4. Using the BIAcore S200, FH1-4 (red line) and I340T FI (green line) were injected at 125nM individually and in combination (blue line) onto a 1000RU C3b coupled CM5 chip surface. I340T FI did not display synergy with FH1-4 indicating no or minimal formation of the AP regulatory TMC.
