Figure 13.

SPR reveals I340T FI inhibits formation of the AP regulatory TMC. To build an AP regulatory TMC 125nM of inactive WT FI and 125nM of FH CCP 1-4 was injected onto a C3b thiol coupled (1000RU) CM5 chip (solid line). A further TMC was built by injection of 125nM of Inactive WT FI, 250nM of I340T FI and 125nM of FH CCP 1-4 onto the same thiol coupled chip (dotted line). Addition of the I340T FI protein resulted in an ~25% lower maximum RU compared to inactive WT FI only as measured by SPR on the Biacore S200. SPR assays are representative of at least 2 independent experiments.