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TMC building SPR assay for FH1-4 variant analysis. Each inactive FI variant (S525A+R406H/K441R/P553S/WT) was injected at 62.5nM with 118nM of FH CCP1-4 onto a C3b (1000RU) thiol -coupled CM5 chip surface and analysis by SPR revealed real-time binding of the AP regulatory TMC for each variant and the WT. SPR Figures are representative of at least 2 repeats.
