Table 1.
Novel viruses discovered in this study
| No. | Virus | ORF No. | Host | Genome length | Classification | Query domain | Identity (%) | Subject virus name | Sequencing depth (X)a | Abundance (RPKM)a | GenBank accession |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Positive-sense single-stranded RNA viruses | |||||||||||
| 1 | Apis picorna-like virus 1 | 2 | A. mellifera | 8530 | Marnaviridae | RdRp | 56.80 | Beihai picorna-like virus 44 | 33 | 0.95 | MZ822067 |
| 2 | Apis picorna-like virus 2 | 2 | A. mellifera | 8588 | Marnaviridae | RdRp | 59.29 | Shahe picorna-like virus 2 | 47 | 0.92 | MZ822068 |
| 3 | Varroa dicistrovirus 1 | 1 | V. destructor | 10319 | Dicistro-related clade | RdRp | 49.68 | Weivirus-like virus sp | 396 | 16.89 | MZ822069 |
| 4 | Apis dicistrovirus 2 | 2 | A. cerana | 8986 | Dicistro-related clade | RdRp | 54.98 | Dicistroviridae sp. | 44 | 1.01 | MZ822070 |
| 5 | Apis dicistrovirus 3 | 2 | A. mellifera | 9068 | Dicistro-related clade | RdRp | 63.01 | Dicistroviridae sp. | 334 | 12.13 | MZ822071 |
| 6 | Apis dicistrovirus 4 | 2 | A. mellifera | 8934 | Dicistro-related clade | RdRp | 64.21 | Wenzhou picorna-like virus 34 | 92 | 2.04 | MZ822072 |
| 7 | Varroa dicistrovirus 2 | 2 | V. destructor | 8844 | Dicistro-related clade | RdRp | 51.67 | Dicistroviridae sp. | 414 | 14.90 | MZ822073 |
| 8 | Apis iflavirus 1 | 1 | A. mellifera | 10521 | Iflaviridae | RdRp | 53.18 | Deformed wing virus | 352 | 11.48 | MZ822075 |
| 9 | Apis iflavirus 2 | 1 | A. mellifera | 9356 | Iflaviridae | RdRp | 55.10 | Picornavirales sp | 22 | 0.73 | MZ822076 |
| 10 | Varroa picorna-like virus | 1 | V. destructor | 7932 | Picorna-related clade | RdRp | 34.70 | Wuhan house centipede virus 3 | 20 | 0.54 | MZ822077 |
| 11 | Apis picorna-like virus 3 | 1 | A. cerana | 10702 | Picorna-related clade | RdRp | 55.23 | Blacklegged tick picorna-like virus 1 | 277 | 5.31 | MZ822078 |
| 12 | Apis picorna-like virus 4 | 1 | A. cerana / A. mellifera | 10033 | Picorna-related clade | RdRp | 53.66 | Wuhan insect virus 12 | 32,978 | 4277.53 | MZ822079- MZ822094 |
| 13 | Apis picorna-like virus 5 | 1 | A. cerana | 10503 | Picorna-related clade | RdRp | 69.67 | Solenopsis invicta virus 7 | 3,405 | 86.55 | MZ822095 |
| 14 | Apis picorna-like virus 6 | 2 | A. cerana | 11354 | Picorna-related clade | RdRp | 39.22 | Hubei picorna-like virus 52 | 53 | 1.68 | MZ822096 |
| 15 | Apis nora virus 2 | 5 | A. mellifera | 10751 | Picorna-related clade | RdRp | 50.55 | Helicoverpa armigera Nora virus | 146 | 5.50 | MZ822097 |
| 16 | Apis polycipivirus | 5 | A. cerana | 11714 | Polycipiviridae | RdRp | 68.76 | Shuangao insect virus 4 | 54 | 1.22 | MZ822098 |
| 17 | Apis virga-like virus | 3 | A. mellifera | 10401 | Virga-related clade | RdRp | 81.50 | Allermuir Hill virus 2 | 436 | 7.25 | MZ822100 |
| 18 | Apis hypovirus 1 | 1 | A. mellifera | 13685 | Hypoviridae | polyprotein | 80.19 | Fusarium sacchari hypovirus 1 | 142 | 2.58 | MZ822101 |
| 19 | Apis hypovirus 2 | 1 | A. mellifera | 11061 | Hypoviridae | polyprotein | 54.85 | Wuhan insect virus 14 | 30 | 0.73 | MZ822102 |
| 20 | Apis hypovirus 3 | 1 | A. mellifera | 11285 | Hypoviridae | polyprotein | 53.98 | Wuhan insect virus 14 | 396 | 4.34 | MZ822103 |
| Negative-sense single-stranded RNA viruses | |||||||||||
| 21 | Apis rhabdovirus 3 | 6 | A. cerana | 13131 | Rhabdoviridae | RdRp | 45.11 | Wuhan Fly Virus 2 | 89 | 3.92 | MZ822104 |
| 22 | Apis rhabdovirus 4 | 5 | A. cerana | 13489 | Rhabdoviridae | RdRp | 49.72 | Diachasmimorpha longicaudata rhabdovirus | 344 | 8.45 | MZ822105 |
| 23 | Apis rhabdovirus 5 | 5 | A. mellifera | 13489 | Rhabdoviridae | RdRp | 50.17 | Diachasmimorpha longicaudata rhabdovirus | 67 | 2.42 | MZ822106- MZ822108 |
aThe largest one is shown if detected in multiple libraries
RPKM reads per kilobase million