Table 2.
Selected publications of fragment‐based MS screening assays.
| Reactive fragment | Warhead | Target protein | MS platform | Citation |
|---|---|---|---|---|
| Cys‐reactive | Acrylate | Pappain | LC‐TOF | Kathman et al (2014) |
| Cys‐reactive | α,β‐unsaturated methyl ester | HOIP | LC‐TOF | Johansson et al (2019) |
| Cys‐reactive | Chloroacetamide | Pin1 | LC‐TOF | Dubiella et al (2021) |
| Cys‐reactive | Acrylate | BRD domains | LC‐Ion Trap | Olp et al (2020) |
| Cys‐reactive | Acrylamide | KRas/KRas(G12C) | LC‐TOF | Ostrem et al (2013) |
| Lys‐reactive | Acrylate/alpha‐beta unsaturated esters | HSP72 | LC‐qTOF and LC–MS/MS | Pettinger et al (2017) |
| Lys‐reactive | Aryl boronic acid carbonyl | Mcl‐1 | LC‐TOF | Akçay et al (2016) |
| PhABit | Alkyl diazirine/alkyne tag | BRD4/KRas | RapidFire‐TOF | Grant et al (2020) |
| PhABit | 5× photoreactive groups | CDK(2/7/9) | LC‐TOF | Grant et al (2019) |
| PhABit | Diazirine | CA(ii) | RapidFire‐TOF | Thomas et al (2021) |
| Tyr‐reactive | Sulfonyl‐fluoride | GST's | LC–MS/MS | Shishido et al (2017) |
| Ser‐reactive | Aryl fluorosulfate | DcpS | LC‐TOF/LC–MS/MS | Fadeyi et al (2017) |