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. 2022 Dec 1;15(1):e15631. doi: 10.15252/emmm.202115631

Figure EV2. Dependency of IMPDH1 and IMPDH2 in human AML cells.

Figure EV2

  • A, B
    Relative mRNA expression of IMPDH1 (A) and IMPDH2 (B) in PDX cells derived from patients with MLL‐fusion or non‐MLL‐fusion AMLs.
  • C
    MLL‐AF9‐expressing CB cells and MOLM13 cells were transduced with Cas9 together with non‐targeting (NT) sgRNA or IMPDH1‐targeting sgRNAs (IMPDH1‐KO1 and KO2) co‐expressing tRFP657. Changes in the frequency of tRFP657+ cells (sgRNA‐transduced cells) in cell cultures are shown. Results are normalized to the frequency of tRFP657+ cells at day 4, set to 1.
  • D
    The tRFP657+ cells were sorted and subjected to western blotting.

Source data are available online for this figure.