FIG. 3.

Effect of various inhibitors on internalization into INT407 cells of C. jejuni 81-176 (□) and the mutant virB11::Km strain (■). At 1 h prior to the addition of bacteria to the monolayer, the epithelial cells were incubated either with no inhibitor (ni) or with 1 μM cytochalasin D (CD), 10 μM colchicine (Co), 1 μM demecolcine (De), 20 μM nocodazole (No), or 50 μM taxol (Ta). Each inhibitor was maintained throughout the 2-h invasion period. The relative percent invasiveness was determined as 100 multiplied by recovery in the presence of inhibitors divided by recovery in the absence of inhibitors (i.e., 100% relative invasiveness). S. enterica serovar Typhi Ty2W served as an MF-dependent invasive control. No other inhibitor showed a significant effect on S. enterica serovar Typhi internalization. Results are presented as the mean of at least three separate experiments and standard error, shown as bars above or below the mean.