Figure 3.

Cytotoxic effect of PFEE on HeLa cell at (a) 24 h, (b) 48 h, (c) 72 h. in vitro cytotoxic effect of PFEE (0–1250 μg/ml was measured by MTT assay on HeLa cell line. Bar graphs shows % of viable cells (Y axis) against PFEE concentration (X axis). (a-c) demonstrates decreasing percentage of viable cells with increasing concentration of PFEE as compared to untreated cell. Data are representative of three independent experiment and bar graph showed mean ±SEM (**P < 0.05, ***P < 0.001, ns not significant)