| Antibodies |
|
| Mouse monoclonal anti-FLAG M2 Alkaline Phosphatase |
Sigma-Aldrich |
Cat#A9469; RRID: AB_439699 |
|
| Bacterial and virus strains |
|
|
E. coli BL21-CodonPlus (DE3)-RIL (chemocompetent) |
Agilent |
Cat#230245 |
|
| Chemicals, peptides, and recombinant proteins |
|
| Pmel |
NEB |
Cat#R0560L |
| Yeast nitrogen base (YNB) without amino acids and ammonium sulfate |
Sigma-Aldrich |
Cat#Y1251 |
|
15N-labeled ammonium sulfate |
CIL |
Cat#NLM-713-50 |
| Deuterium oxide (2H2O) |
CIL |
Cat#DLM-4-99.8 |
| Theophylline |
Sigma-Aldrich |
Cat# T1633 |
| Terrific Broth (TB) |
RPI |
Cat#T15000–1000.0 |
| Biotin |
Sigma-Aldrich |
Cat#B4639 |
| lodoacetamide |
Sigma-Aldrich |
Cat#I1149 |
| Lauryl maltose neopentyl glycol (LMNG) |
Anatrace |
Cat#NG310 |
| Cholesteryl hemisuccinate (CHS) |
Anatrace |
Cat#CH210 |
| Adenosine 5’-triphosphate (ATP) |
Sigma-Aldrich |
Cat#A2383 |
| ZM241385: 4-(2-[7-Amino-2-(2-furyl)[1,2,4]triazolo[2,3-a] [1,3,5]triazin-5-ylamino]ethyl)phenol |
Tocris |
Cat#1036 |
| NECA: 5’-N-ethylcarboxamidoadenosine |
Tocris |
Cat#1691 |
| Guanosine 5’-diphosphate (GDP) |
Sigma-Aldrich |
Cat#G7127 |
| Apyrase |
NEB |
Cat#M0398S |
|
| Experimental models: Organisms/strains |
|
|
P. pastoris BG12 (electrocompetent) |
BioGrammatics |
Cat#PS004-01 |
|
| Recombinant DNA |
|
| pPIC9K-A2AAR |
Eddy et al., 201836
|
N/A |
| pPIC9K-A2AAR-W2466.48F |
Eddy et al., 201836
|
N/A |
| pET15b-Mini-Gs |
Carpenter et al., 201630
|
N/A |
|
| Software and algorithms |
|
| Topspin version 3.6.3 |
Bruker |
https://www.bruker.com/en/products-and-solutions/mr/nmr-software.html
|
|
| Other |
|
| TALON resin |
Clontech |
Cat#635504 |
| Ni-NTA resin |
ThermoFisher |
Cat# 88222 |
| His-Trap HP NiNTA 5 mL column |
Cytiva |
Cat#17524802 |
| PD-10 |
Cytiva |
Cat#17085101 |
| PD MiniTrap G25 column |
Cytiva |
Cat#28918007 |
| HiPrep 26/10 column |
Cytiva |
Cat#17508701 |
| HiLoad Superdex 75 column |
Cytiva |
Cat#90100805 |
| Increase Superdex 200 10/300 GL column |
Cytiva |
Cat#28990944 |
