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. Author manuscript; available in PMC: 2023 Dec 11.
Published in final edited form as: Sci Immunol. 2022 Dec 2;7(78):eadd3075. doi: 10.1126/sciimmunol.add3075

Fig 2. Comparing routes of immunization reveals that intranasal prime-boost vaccination induces more CD103+ CD8 T cells in the lung parenchyma.

Fig 2.

(A) Mice were either IM, IV or IN prime-boosted and spleen, iliac LN, medLN and lungs were examined ≥28 days post boost. (B) Quantification of antigen-specific CD8+ T cells in the indicated SLOs. (C-D) Representative flow cytometry plot (C) and quantification (D) of NP366-specific CD8+ CD69+CD62L− SLO TRM. (E) Quantification of antigen-specific CD8+ T cells in the indicated lung compartments. (F-G) Representative flow cytometry plot (F) and quantification (G) of NP366+ CD8 T cell subsets in the lung parenchyma (IV-neg). Data represent N = 2 independent experiments with n = 4-5 mice per group. Data are shown as mean ± SEM. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 as determined by one-way ANOVA and Tukey’s multiple comparisons test.