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. Author manuscript; available in PMC: 2023 Dec 11.
Published in final edited form as: Sci Immunol. 2022 Dec 2;7(78):eadd3075. doi: 10.1126/sciimmunol.add3075

Fig 5. Combining intramuscular immunizations with an intranasal mRNA boost achieves high levels of both circulating memory and lung TRM.

Fig 5.

(A) IM prime-boosted mice received a second booster via IN route and spleen, iliac LN, medLN and lungs were examined ≥28 days post secondary boost. (B-C) Quantification of antigen-specific CD8+ T cells (B) and CD4+ T cells (C) in the indicated compartments. (D-E) Representative flow cytometry plot (D) and quantification (E) of IV-neg NP366-specific CD8+ T cell subsets in the lung. (F-G) Representative flow cytometry plot (F) and quantification (G) of IV-neg NP261-specific CD4+ T cell subsets in the lung. Data represent N = 2 independent experiments with n = 4-5 mice per group. Data are shown as mean ± SEM. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 as determined by unpaired two-tailed Student’s t test.