Table 2. Summary of methods for mapping connectional anatomy.

Each method is listed with the section(s) of this review where it is discussed (dMRI: diffusion-weighted magnetic resonance imaging; PLI: polarized light imaging; PS-OCT: polarization-sensitive optical coherence tomography; CLARITY: clear lipid-exchanged acrylamide-hybridized rigid imaging/immunostaining/in situ hybridization-compatible tissue-hydrogel; CT: computed tomography; EM: electron microscopy). *: Needed in the human brain. **: Needed in transmission but not block-face scanning mode.

		Versatility		Ease of processing		See §
	Scale	Feasible in vivo	Feasible in human	Tissue staining needed	Section registration needed
dMRI						2
Klingler’s dissection						4.2
PLI						5.2, 6
PS-OCT						5.2, 6
CLARITY					*	5.2, 6
Anatomic tracing						4.1
Myelin staining						5.1, 6
Micro-CT						6
EM					**	6