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. 2022 Dec 22;8(1):ysac032. doi: 10.1093/synbio/ysac032

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© The Author(s) 2023. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Homology-dependent one-pot IVA with AssemblyTron. (A) AssemblyTron one-pot IVA was verified by amplification of appropriate bands via gel electrophoresis. Fragments 1 and 2 are indistinguishable due to size similarity; however there is a slight resolution between Fragments 1 and 3. (B) Consistent transformation efficiency and sequence verification validate the one-pot IVA protocol.