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. 2022 Dec 28;2(1):pgac300. doi: 10.1093/pnasnexus/pgac300

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The Author(s) 2022. Published by Oxford University Press on behalf of National Academy of Sciences.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 6. — siRNA silencing of genes critical to GTme growth. (A) Experimental design for gene silencing and proliferation assay in isolated GTme cells. (B and C) Growth curves of GTme cells reverse-transfected with siRNA against Ar, Gli3, and Mafb. *P < 0.01, siRNA vs. mock of same sex; **P < 0.05. (D) Real-time qPCR confirming efficient knockdowns of respective genes. Note the sex-dimorphic expression of Ar in the control GTme cells (male 0.9987 ± 0.0526 vs. female 0.3290 ± 0.0568, N = 4, P = 0.0001).