FIG. 3.

Production of IL-12 p70 and NO in vitro by splenic macrophages from uninfected and P. chabaudi AS-infected WT and GKO mice. Splenic macrophages from uninfected and infected (day 2 for IL-12 p70 and day 7 for NO) mice were cultured for 20 h in the presence of 1 μg of LPS/ml. Supernatants were collected, and the levels of IL-12 p70 (A) and NO₂⁻ (B) were determined. Data are means ± SEM of three individual mice per group from one of two experiments. Statistically significant differences are indicated as follows: †, P < 0.05; ‡, P < 0.01; §, P < 0.001 (uninfected versus infected mice); ∗, P < 0.05; ∗∗, P < 0.01 (WT versus GKO mice).