Table 1.
Quantification of SARS-CoV-2 RNA in body fluids and tissues by ddPCR.
| Blood | BAL pre-/peritransplant | BAL posttransplant day 1 | Left lung |
Right lung |
Distal trachea | Left bronchus | Right bronchus intermedius | Lymph node |
||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Upper lobe | Lower lobe | Upper lobe | Middle lobe | Lower lobe | Tracheal | Thoracic node 1 | Thoracic node 2 | |||||||
| Recipient/explant | – | – | – | 0.0104 | 0.044 | 0.0042 | 0.0108 | – | n/a | – | 0.0079 | n/a | n/a | n/a |
| Donor | – | – | n/a | n/a | n/a | 0.0099 | – | n/a | – | n/a | n/a | 0.0054 | – | 0.006 |
Detectable RNA is presented as the average nucleocapsid gene copies per ng of RNA added to the assay. “–” indicates a negative result under the limit of detection of the assay. “n/a” indicates that the tissue was not available for ddPCR.
BAL, bronchoalveolar lavage; ddPCR, droplet digital polymerase chain reaction.