Expression of sedoheptulose 1,7‐bisphosphatase/fructose 1,6‐bisphosphatase and cytochrome C6 in transgenic tobacco improves water use efficiency and biomass in field‐grown plants. (a) Transgenic tobacco plants in field plots in Illinois. (b) Intrinsic water use efficiency and (c) biomass are increased in field‐grown transgenic plants expressing the bifunctional sedoheptulose 1,7‐bisphosphatase/fructose 1,6‐bisphosphatase (SBPase/FBPase) enzyme together with the algal cytochrome C6. CN, wild‐type and azygous controls; A/gs, assimilation rate over stomatal conductance; C, cytochrome C6; iWUE, intrinsic water use efficiency; PPFD, photosynthetic photon flux density; SB, SBPase/FBPase (Lopez‐Calcagno et al., 2020). In (b), asterisks indicate significant differences between the transgenic plants and the controls, using a linear mixed‐effects model and type III analysis of variance (ANOVA) and contrast analysis (*, P < 0.05; **, P < 0.01; ***, P < 0.001); in (c), asterisks indicate significance between transgenic plants and controls, or between genotypes, using ANOVA with Tukey's post‐hoc test (*, P < 0.05; **, P < 0.01, ***, P < 0.001). This figure is modified from figures presented in Lopez‐Calcagno et al. (2020).