Table 1.
Primers | Sequence (5’-3’) |
---|---|
GA F1/12S | CACAAAGGTTTGGTCCTGACTTTAC |
GA R1/16S | GGGCTCTGCCACCTTAACATGY |
GA F2/16S | CTAGTACGAAAGGACCGAGAAGATGA |
GA R2/COI | TGTGTGATAAGGRGGMGGGCAGC |
GA F3/COI | CGACGRTACTCAGACTACCCHGA |
GA R3/ND4L | TTGTAGGAGRTTTARGCTYTGVAGG |
GA F4/ND4L | CACCGAACCCACCTWCTCTCYGC |
GA R4/Cytb | GCAATTTTTAGTARKGGGTGKGTTTT |
GA F5/Cytb | GCCAGGACTYTAACCAGGACTAATG |
GA R5/12S | GCGGTGGCTGGCACGAGTTTTAC |
The five pairs of primers (GA F1/12S and GA R1/16S, GA F2/16S and GA R2/COI, GA F3/COI and GA R3/ND4L, GA F4/ND4L and GA R4/Cytb, GA F5/Cytb and GA R5/12S) were designed for the mitogenome amplification and Sanger sequencing.