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. Author manuscript; available in PMC: 2023 Jan 11.
Published in final edited form as: Nat Aging. 2022 Oct 12;2(10):923–940. doi: 10.1038/s43587-022-00290-0

Extended Data Fig. 5. Validation of splicing deficient transcripts of synaptic pathway in N40K-Tg mice.

Extended Data Fig. 5.

a-b, Quantitation of intron accumulation at the selected region of Gabra2, Gng7, Kcnh1, and Camk1d. IGV software was used to display RNA read density with same scale range in each gene from RNA-seq (ex: exon; in: intron). Red boxes show selected regions for quantification. Intron reads % was analyzed by RNA-seq ((intron-exon junctions + introns)/(intron-exon junctions + introns + exons + exon-exon junctions)). Intron retention % was analyzed by RT-PCR ((intron-containing PCR band intensity)/(intron-containing PCR band intensity + exon-exon PCR band intensity)). Gabra2: intron 6 vs exon 6 and 7; Gng7: intron 2 vs exon 2 and 3, Kcnh1: intron 9 vs exon 9 and 10, and Camk1d: intron 5 vs exon 5 and 6. 12-month-old: WT n = 3, Tg n = 3. Scale bar, 10 kb. Data are shown as mean ± SEM. Statistical significance was analyzed by Student's t-test. Data are shown as mean ± SEM, * p < 0.05; ** p < 0.01; *** p < 0.001.