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. 2022 Dec 22;11:e82240. doi: 10.7554/eLife.82240

Figure 5. Amplicon deep sequencing of P0 in pooled evolved populations.

(A) (Left panel) Number of reads carrying a P0 sequence with two adaptive SNPs 30 and 37 bp upstream of galK, respectively (‘T>A + C>T’ in blue) or its respective single SNPs (‘T>A’ in green, ‘C>T’ in cyan). Values are normalized to the number of reads with ancestral P0 for IS- and IS+ populations evolved in 0.01% galactose. The mean fraction of reads with any single SNP in galK is shown as a control (grey). Error bars represent the standard deviation of three replicates, consisting each of 32 pooled evolved populations. (Right panel) Read fractions of the same respective SNPs shown for a pool of all 96 IS+ and IS- populations evolved in the absence of galactose. (B) Mean read fractions as in (A) shown for three replicates of each 32 pooled populations evolved in intermediate (0.1%) galactose.

Figure 5.

Figure 5—figure supplement 1. Total number of sequencing reads for all replicates.

Figure 5—figure supplement 1.

(A) Log plot of total read numbers showing contamination of P0 amplicons with P02 amplicons stemming from pooled samples of the 0.1% galactose populations of both promoter sequences (blue rectangles; see Methods).