Strain name	Genotype	Purpose	Source
MG1655	F- λ- ilvG- rfb-50 rph-1	Strain background for all evolution experiments	Lab collection
IT013-TCD	BW27784, JA23100::galP, mglBAC::FRT, galK::FRT, locus1::pBAD-galK	Strain with pBAD-galK for testing expression-growth relation	Tomanek et al., 2020
BW25142	lacIq rrnB3 ∆lacZ4787 hsdR514 ∆(araBAD)567 ∆(rhaBAD)568 ∆phoBR580 rph-1 galU95 ∆endA9 uidA(∆MluI)::pir-116 recA1	Host for pir plasmid pMS6*	Khlebnikov et al., 2001
MS022	MG1655, JA23100::galP, mglBAC::FRT, galK::FRT	IS+ background for ancestor strain construction	Lab collection
IT030	MS022 locus2::P0-RBS-galK -RBS-yfp -FRT-pR-cfp	IS+ ancestor strain	Tomanek et al., 2020
IT049	MS022 deleted for IS1C	IS- background for ancestor strain construction	This study
IT049-P0	IT049 locus2::P0-RBS-galK -RBS-yfp -FRT-pR-cfp	IS- ancestor strain P0	This study
IT049-P0-1	IT049 locus2::P0-1-RBS-galK -RBS-yfp -FRT-pR-cfp	IS- ancestor strain P0-1	This study
IT049-P0-2	IT049 locus2::P0-2-RBS-galK -RBS-yfp -FRT-pR-cfp	IS- ancestor strain P0-2	This study
IT049-P0-3	IT049 locus2::P0-3-RBS-galK -RBS-yfp -FRT-pR-cfp	IS- ancestor strain P0-3	This study
MS022-P0	MS022 locus2::P0-RBS-galK -RBS-yfp -FRT-pR-cfp	IS+ ancestor strain P0	This study
MS022-P-01	MS022 locus2::P0-1-RBS-galK -RBS-yfp -FRT-pR-cfp	IS+ ancestor strain P0-1	This study
MS022-P0-2	MS022 locus2::P0-2-RBS-galK -RBS-yfp -FRT-pR-cfp	IS+ ancestor strain P0-2	This study
MS022-P0-3	MS022 locus2::P0-3-RBS-galK -RBS-yfp -FRT-pR-cfp	IS+ ancestor strain P0-3	This study
IT030-H5r	MS022 locus2::pconst-RBS-galK -RBS-yfp -FRT-pR-cfp	Strain with constitutive galK expression conferred by two SNPs in P0	Tomanek et al., 2020
IT030-D8c	MS022 locus2::pconst-RBS-galK -RBS-yfp -FRT-pR-cfp	Strain with constitutive galK expression conferred by one SNP in P0	Tomanek et al., 2020