Name	Sequence	Purpose
E_flank_f	GCTGGAGCCACTTGTAGCC	cassette integration test locus 2, sequencing P0s
E_flank_r	TCCTTGCTGAATCATTTTGTTC	cassette integration test locus 2
P0_check_Fw	GTGTGAGTGGCAGGGTAG	sequencing P0s (together with E_flank_f)
qPCR_galK _Fw	GCTACCCTGCCACTCACA	estimating galK copy number
qPCR_galK _Rv	CGCAGGGCAGAACGAAAC	estimating galK copy number
rbsB_qPCR_Fw	GGCACAAAAATTCTGCTGATTAA	qPCR control locus
rbsB_qPCR_Rv	GCAGCTCGATAACTTTGGC	qPCR control locus
P1_P0-1	GCCTTAGTTGTAAGTGTCTACCAT GTCCCCGAACAAGTGTTCACTATG TCTAGGCCCGCACGCAAGAC	integration of the selection and reporter cassette with P0-1 (Fw primer)
P1_P0-2	GCCTTAGTTGTAAGTGTCTACCAT GTCCCCGAACAAGTGTTCACTATG TCTCGGGGGGACAGCAGCG	integration of the selection and reporter cassette with P0-2 (Fw primer)
P1_P0-3	GCCTTAGTTGTAAGTGTCTACCAT GTCCCCGAACAAGTGTTCACTATG TCTGTATTTGGGATGCGGGTAGTAGA	integration of the selection and reporter cassette with P0-3 (Fw primer)
E_int_Rv	TCGGAAGGGAAGAGGGAGTGCGGG AAATTTAAGCTGGATCACATATTGCC GAGGCCTTATGCTAGCTTC	integration of the selection and reporter cassette (Rv primer)
E_int_Fw	GCCTTAGTTGTAAGTGTCTACCATGTC CCCGAACAAGTGTTCACTATGTCACCG GAAAGACGGGCTTC	integration of the selection and reporter cassette with P0 (Fw primer)
deep_seq_Fw	TCGTCGGCAGCGTCAGATGTGTATAAG AGACAGACGGGTTCTTATGCCTTAGTT	1st step PCR for amplicon deep sequencing (with 5´nextera anchor for Illumina sequencing)
deep_seq_Rv	GTCTCGTGGGCTCGGAGATGTGTATAA GAGACAGGTGTGAGTGGCAGGGTAG	1st step PCR for amplicon deep sequencing (with 5´nextera anchor for Illumina sequencing)