KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rat anti mouse CD45 BUV395 clone 30-F11 | BD | Cat# 565967; RRID: AB_2651134 |
| Rat anti mouse CD38 FITC clone 90 | Invitrogen | Cat# 11-0381-82; RRID: AB_465024 |
| Rat anti mouse CCR2 PE clone SA3203G11 | BioLegend | Cat# 150610; RRID: AB_2616982 |
| Rat anti mouse CD11b PE-Cy7 clone M1/70 | Invitrogen | Cat# 25-0112-82; RRID: AB_469588 |
| Rat anti mouse CD64 APC clone X54-5/7.1 | BioLegend | Cat#139306; RRID: AB_11219391 |
| Rat anti mouse CD45 eFluor450 clone M1/69 | ThermoFisher Scientific | Cat# 48-0242-82; RRID: AB_1311169 |
| Rat anti mouse Ly6C clone AL-21 | BD | Cat# 563011; RRID: AB_2737949 |
| Rat anti mouse CD11c BV711 clone HL3 | BD | Cat# 563048; RRID: AB_2734778 |
| Rat anti mouse Ly6G AF700 clone 1A8 | BD | Cat# 551459; RRID: AB_394206 |
| Rat anti mouse Siglec-F PerCP-Cy5.5 clone E50-2440 | BD | Cat# 565526; RRID: AB_2739281 |
| Rat anti mouse I-A/I-E (MHC-II) APC/Fire 750 clone M5/114.15.2 | BioLegend | Cat# 107652; RRID: AB_2616729 |
| Rat anti mouse CD4 PerCP-Cy5.5 clone RM4-5 | BD | Cat# 561115; RRID: AB_393977 |
| Rat anti mouse CD8a FITC clone 53–6.7 | BioLegend | Cat# 100706; RRID: AB_312745 |
| Rat anti mouse CD45R/B220 PE-Cy7 clone RA3-6B2 | BD | Cat# 552772; RRID: AB_394458 |
| mouse anti-alpha smooth muscle actin clone 1A4 | Abcam | Cat# ab7817; RRID: AB_2865422 |
| rabbit mAB anti-SMAD2 clone D43B4 | Cell Signaling | Cat# 5339 |
| rabbit mAB anti-phospho-SMAD2 (Ser465/467) clone 138D4 | Cell Signaling | Cat# 3107 |
| rabbit anti-VINCULIN | Cell Signaling | Cat# 4650 |
| rabbit anti-p44/42 MAPK (Erk1/2) clone 137F5 | Cell Signaling | Cat# 4695 |
| rabbit anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) clone D13.14.4E | Cell Signaling | Cat# 4370 |
| rabbit mAB anti-CXCL2 clone 16H3L10 | invitrogen | Cat# 701126 |
| mouse anti-CCR2 clone 3G7 | Novus Biologicals | Cat# NBP2-35334 |
| mouse anti-CCR2 clone 7A7 | abcam | Cat# ab176390 |
| FcBlock | BD | Cat# 553141; RRID: AB_394656 |
| goat anti-rabbit IgG-HRP | Santa Cruz | Cat# sc-2004 |
| mouse anti-alpha smooth muscle Actin (aSMA) clone 1A4 | Abcam | Cat# ab7817 |
| rabbit Cy5 Tyramide | Akoya | Cat# SAT705A001 EA |
| Biotin Tyramide | Akoya | Cat# NEL705A001KT |
| rabbit envision secondary antibody | Agilent/Dako | Cat# K4001-8 |
| mouse envision secondary antibody | Agilent/Dako | Cat# K4005 |
| Alexa 750-Streptavidin | Thermofisher/Life Technologies | Cat# S21384 |
| Chemicals, peptides, and recombinant proteins | ||
| cOmplete Mini EDTA-free protease inhibitor cocktail | Roche | Cat# 11836170001 |
| PBS | Gibco | Cat# 10010023 |
| UltraPure Low Melting Point agarose | Invitrogen | Cat# 16520–050 |
| Lipopolysaccharides from Pseudomonas aeruginosa | Millipore Sigma | Cat# L9143 |
| CCR2 inhibitor RS 102895 | Cayman Chemical | Cat# 1173022-16-6 |
| Cell Lysis Buffer | Cell Signaling | Cat# 9803 |
| PhosSTOP | Roche | Cat# 4906837001 |
| 4–15% Mini PROTEAN TGX Gels | Bio-Rad | Cat# 4561086 |
| ECL Plus Western blotting system | GE Healthcare | Cat# RPN2132 |
| Superscript II Reverse Transcriptase | ThermoFisher | Cat# 18064–022 |
| RBC lysis buffer | eBioscience | Cat# 00-4300-54 |
| Critical commercial assays | ||
| active TGF-beta ELISA kit | BioLegend | Cat# 437707 |
| total TGF-beta ELISA kit | BioLegend | Cat# 436707 |
| V-PLEX Proinflammatory Panel 1 (mouse) | Mesoscale Discovery | Cat# K15048G-1 |
| V-PLEX Cytokine Panel 1 (mouse) | Mesoscale Discovery | K15245D-1 |
| Lung dissociation kit (mouse) | Mitenyi Biotec | Cat# 130-095-927 |
| miRNEasy Micro kit | Qiagen | Cat# 217084 |
| Agilent RNA6000 Pico Kit | Agilent | Cat# G2938-90046 |
| SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing | Takara Bio | Cat# 634890 |
| Nextera XT DNA Sample Preparation kit | Illumina | Cat# FC-131-1096 |
| miScript II RT kit | Qiagen | Cat# 218161 |
| Mouse CXCL2/MIP-2 DuoSet ELISA | R&D systems | Cat# DY452-05 |
| Deposited data | ||
| scRNAseq data | Schupp et al.26 | GEO: GSE145360 |
| Bulk RNAseq data | GEO | GEO: GSE217102 |
| Experimental models: Organisms/strains | ||
| Cftr knockout mice B6.129P2-KOCftrtm1UNC | Jackson Laboratory | Stock No. 002196 |
| CCR2 knockout mice B6.129S4-Ccr2tm1Ifc/J | Jackson Laboratory | Stock No. 004999 |
| Ccr2cre mice | Dr. Tobias Hohl; Heung and Hohl77 | N/A |
| cftrflox/flox mice | CF animal core facility at Case Western Reserve University | N/A |
| CD45.1 mice B6.SJL-Ptprca Pepcb/BoyJ | Jackson Laboratory | Stock No. 002014 |
| Software and algorithms | ||
| FACSDiva | BD | N/A |
| FlowJo | BD | N/A |
| Prism | GraphPad | N/A |
| enrichR package | https://maayanlab.cloud/Enrichr | N/A |
| edgeR package | https://bioconductor.org | N/A |
| Other | ||
| Nebulizer Pulmo-Aide Compressor | Natallergy | Cat# DRV5650D |