FIG. 2.
Bacterial proliferation and normalized TNF-α secretion from mouse macrophage–viable-bacterium cocultures. Thioglycolate-elicited C3Heb/FeJ mouse macrophages were cocultured with 5 × 105 CFU of E. coli O111:B4 or S. aureus M/ml. (a) Bacterial proliferation was determined at the times indicated; (b) TNF-α concentration from Fig. 1a normalized to bacterial number from panel a. Data are means ± SEM. ∗, P < 0.05; ∗∗, P < 0.005. Three independent experiments were performed. For the data lacking error bars, variation was too small to indicate.