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. 2022 Dec 21;613(7943):332–339. doi: 10.1038/s41586-022-05550-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 6 — a. Flowchart for the sub-tomogram averaging strategy of the ribosome. For details see Methods. b. Gold-standard FSC curve of the sub-tomogram average. c. Scheme of the ribosome identification workflow. For details see Fig. 3 and Methods. d. Multiple sequence alignment of expansion segments (ESs) 9 and 39 of the LSU rRNA of L. ossiferum compared to sequences from T. kodakarensis and those present in the MAGs of the organisms in the enrichment culture (Desulfovibrio_bin1: 44 contigs, 100% complete, 0% contamination; Methanogenium_bin1: 56 contigs, 98.7% complete, 2.6 % contamination; Methanogenium_bin2: 37 contigs, 98.7% complete, 1.9% contamination). The alignment reveals that ESs are only present in L. ossiferum.