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. 2022 Dec 21;613(7943):332–339. doi: 10.1038/s41586-022-05550-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 3 — Amplicons were generated from environmental DNA and submitted to Illumina Miseq (300 PE) sequencing. SILVA was used for taxonomic classification. These analyses show that the LkF and LkR primers designed in this study are highly specific to Asgard archaea 16S rRNA genes.