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. 2022 Nov 11;11(1):e2093. doi: 10.1002/mgg3.2093

FIGURE 1.

FIGURE 1

iNSCs and iNs characterization. (a) Protocol diagram for neuron differentiation. (b) Immunostaining of control and patient iPSC‐derived neural stem cells (iNSCs) showed expression of the markers NESTIN (green) and SOX2 (red). Nuclei were stained with DAPI (blue). Scale bar 50 μm. (c) Immunostaining of control and patient iNs showing expression of neuron markers of DCX (green) and MAP2 (red). Nuclei were stained with DAPI (blue). Scale bar 50 μm. (d) PCR analysis of the GAA repeat region in the FXN gene using genomic DNA extracted from iNSCs and iNs from control and FRDApatient. (e) qPCR quantification of FXN mRNA levels in iNSCs and iNs from control and FRDApatient. Data are expressed as means ± SD of three independent experiments. *p < 0.05. *FA means FRDA.