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. 2022 Nov 11;11(1):e2093. doi: 10.1002/mgg3.2093

FIGURE 5.

FIGURE 5

Frataxin lentivirus transduction in FRDA iCMs and RNA‐seq analysis. (a) qPCR quantification of FXN mRNA levels of transduced FRDA iCMs with different lentivirus dilutions to determine the optimal concentration for transduction (1/10 dilution equivalent to 27 ng/ml of p24). Data are expressed as means ± SD of three independent experiments. *p < 0.05. (b) Cytotoxicity assay to determine if the chosen concentration for transduction was toxic to iCMs. Data are expressed as means ± SD of three independent experiments. *p < 0.05. (c) Venn diagram illustrating the DE genes between transduced and non‐transduced FRDA iCMs. (d) a heatmap illustrating the expression of 417 genes DE in both groups (transduced and non‐transduced FRDA iCMs). Three independent replicates are shown per group (e) enrichment analysis of DE genes in transduced and non‐transduced iCMs using g:Profiler. We included results from the gene ontology and KEGG databases. *FA means FRDA.