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. 2023 Jan 12;4(2):100918. doi: 10.1016/j.xcrm.2023.100918

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© 2023 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

The cross-binding and cross-neutralization of R14 and S43 to SARS-CoV-2 and its variants

(A) The binding kinetics of R14 and S43 to RBDs from SARS-CoV-2 and its variants obtained using a BIAcore 8K system in single-cycle mode. Values summarized in (C) are the mean ± SD of three independent experiments. “-” represents no detectable dissociation from the RBD.

(B and C) (B) Neutralization of R14 and S43 against SARS-CoV-2 and its variant pseudotyped viruses in Vero cells. n = 3 per nanobody concentration points. IC₅₀ values summarized in (C) are the mean ± SD of three independent results.

(D) The binding between R14 or S43 and multiple sarbecoviruses. The binding kinetics of R14 and S43 with RBDs from several sarbecoviruses were obtained using a BIAcore 8K system in single-cycle mode. Human Fc-tagged RBD proteins were captured on the chip surface, and serial dilutions of His-tagged R14 or S43 were then injected over the chip surface. Values summarized in (E) are the mean ± SD of three independent experiments. “-” represents no detectable dissociation from the RBD. “×” represents no detectable binding to the RBD.