REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
CoVIC antibodies (CoVIC 1 to CoVIC 397)	This study and Hastie and Li et al.15	https://covic.lji.org
Bacterial and virus strains
VSV-ΔG-GFP	Karafast	Cat# EH1020
SARS-CoV-2 D614G/Vesicular Stomatitis Virus pseudovirus	This study	N/A
SARS-CoV-2 Beta/Vesicular Stomatitis Virus pseudovirus	This study	N/A
SARS-CoV-2 Delta/Vesicular Stomatitis Virus pseudovirus	This study	N/A
SARS-CoV-2 Mu/Vesicular Stomatitis Virus pseudovirus	This study	N/A
SARS-CoV-2 Omicron BA.1/Vesicular Stomatitis Virus pseudovirus	This study	N/A
SARS-CoV-2 Omicron BA1.1/Vesicular Stomatitis Virus pseudovirus	This study	N/A
SARS-CoV-2 Omicron BA.2/Vesicular Stomatitis Virus pseudovirus	This study	N/A
SARS-CoV-2 Omicron BA2.12.1/Vesicular Stomatitis Virus pseudovirus	This study	N/A
SARS-CoV-2 Omicron BA.4/5/Vesicular Stomatitis Virus pseudovirus	This study	N/A
Chemicals, peptides, and recombinant proteins
Uranyl formate	Electron Microscopy Sciences	Cat# 22451
Papain	Sigma	Cat# P3125
L-cysteine	Calbiochem	Cat# 4400
SARS-CoV-2 D614G Spike protein ectodomain with HexaPro mutations and C-terminal Foldon, HRV3C protease cleavage site, 8x-His-tag, and strep-tag	This study	N/A
SARS-CoV-2 Omicron BA.1 Spike protein ectodomain with HexaPro mutations and C-terminal Foldon, HRV3C protease cleavage site, 8x-His-tag, and strep-tag	This study	N/A
Critical commercial assays
ExpiFectamine CHO Transfection kit	ThermoFisher	Cat# A29129
Deposited data
Negative stain electron microscopy reconstructions of antibodies in complex with SARS-CoV-2 D614G spike	Electron Microscopy Data Bank (http://www.emdataresource.org/)	EMD: 24340, 24341, 24342, 24344, 24353, 24357, 24358, 28090, 28091, 28092, 28093, 28094, 28095, 28096, 28097, 28098, 28099, 28100, 28102, 28103, 28104, 28105, 28106, 28168, 28169, 28170, 28171
Table containing neutralization activity of all items in the CoVIC panel against pseudovirus bearing major SARS-CoV-2 VoCs	Mendeley Data (https://data.mendeley.com/)	Mendeley Data: https://dx.doi.org/10.17632/xjgsyxckt3.1
Experimental models: Cell lines
293T cells	ATCC	Cat# CRL-3216; RRID:CVCL_0063
Vero cells	ATCC	Cat# CRL-1586; RRID:CVCL_0574
ExpiCho-S cells	Thermo Fisher Scientific	Cat# A29127; RRID:CVCL_5J31
Recombinant DNA
Empty vector: phCMV3	Genlantis	Cat# P003300
pCAGGS-VSV-G	Kerfast	Cat# EH1017
phCMV3-D614G Spike	This study	Genbank: QHD43416.1 with D614G mutation
phCMV3-Beta Spike	This study	Genbank: QHD43416.1 with L18F, D80A, D215G, Δ242-244, R246I, K417N, E484K, N501Y, D614G, and A701V mutations
phCMV3-Delta Spike	This study	Genbank: QHD43416.1 with T19R, G142D, E156G, Δ157–158, L452R, T478K, D614G, P681R, and D950N mutations
phCMV3-Mu Spike	This study	Genbank: QHD43416.1 with T95I, Y144S, Y145N, N146ins, R346K, E484K, N501Y, D614G, P681H, and D950N mutations
phCMV3-Omicron BA.1 Spike	This study	Genbank: QHD43416.1 with A67V, Δ69/70, T95I, G142D, Δ143/145, N211I, Δ212, ins214 EPE, G339D, S371L, S373P, S375F, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, D796Y, N856K, Q954H, N969K, and L981F mutations
phCMV3-Omicron BA1.1 Spike	This study	Genbank: QHD43416.1 with A67V, Δ69/70, T95I, G142D, Δ143/145, N211I, Δ212, ins214 EPE, G339D, R346K, S371L, S373P, S375F, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, D796Y, N856K, Q954H, N969K, and L981F mutations
phCMV3-Omicron BA.2 Spike	This study	Genbank: QHD43416.1 with T19I, L24S, Δ25/27, G142D, V213G, ins214 EPE, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, and N969K mutations
phCMV3-Omicron BA.2.12.1 Spike	This study	Genbank: QHD43416.1 with T19I, L24S, Δ25/27, G142D, V213G, ins214 EPE, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452Q, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, S740L, N764K, D796Y, Q954H, and N969K mutations
phCMV3-Omicron BA.4/5 Spike	This study	Genbank: QHD43416.1 with T19I, L24S, Δ25/27, Δ69/70, G142D, V213G, ins214 EPE, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q493, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, and N969K mutations
Software and algorithms
CryoSPARC	CryoSPARC	https://www.cryosparc.com/
GraphPad Prism 9	GraphPad Software	https://www.graphpad.com/
Carterra “Kinetics” and “Epitope” software packages	Carterra	https://carterra-bio.com/
Other
Titan Halo electron microscope	Thermo Fisher Scientific	https://www.thermofisher.com/us/en/home.html
AmMag Ni magnetic beads	GenScript	Cat# L00776
Superose 6 Increase 10/300 GL	GE Healthcare	Cat# 29091596
CellInsight CX5 High Content Screening Platform	Thermo Fisher Scientific	Cat# CX51110
CF400-Cu grids	Electron Microscopy Sciences	Cat# CF400-Cu