Quantitation of cell bound LOS on NTHi strain AAr176p+ after SDS-PAGE and silver staining. Lanes 1 and 7, molecular weight standards; lane 2, 3 × 108 CFU of strain AAr176p+; lanes 3 through 6, 3, 1.5, 0.75, and 0.375 μg, respectively, of purified LOS from strain AAr176p+.