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. 2023 Jan 11;6:29. doi: 10.1038/s42003-023-04416-x

Fig. 6. Vascular permeability is enhanced in Zfp185 knockout mice.

Fig. 6

a, b mZfp185 interacts with PKA regulatory subunits (RIα, RIβ, RIIα, and RIIβ) as well as actin. PKA regulatory subunits (RIα, RIβ, RIIα, and RIIβ)-Flag and HA-mZfp185 are overexpressed in HEK293T cells. Anti-Flag beads were used for coimmunoprecipitation. Representative blots are shown (n = 3). c Generation of Zfp185 knockout mice by CRISPR/Cas9 genome editing technology. The target sequence for Zfp185 gene editing is shown. Sanger sequencing confirms 24794 bp deletion. d, e mZfp185 is detected by anti-ZNF185 antibody. d Myc-mZfp185-HA were overexpressed in the HEK293T cells. Anti-ZNF185 antibody was used to detect overexpressed Zfp185. Representative blots are shown (n = 3). e Anti-ZNF185 antibody detects Zfp185 in the ear in WT mice (n = 3). f Representative images of Evans blue leakage. Inflammatory mustard oil increased Evans blue leakage in Zfp185 knockout mice. Scale bars, 5 mm. g Quantification of Evans blue leakage in ears. Two-sided Student’s t test, **p < 0.01 (n = 3). Data are presented as the mean ± standard error.