Figure 2.

GlycoSense workflow. 1. A fluorescently labeled glycoprotein, or glycoprotein with a fluorescently labeled secondary detection reagent, is incubated with multiplex GlycoSense microspheres in suspension then run on a flow cytometer. 2. Microsphere peaks are determined by red (633/640 nm excitation, 670 nm emission, FL4) fluorescence while bound glycoprotein is detected by green (488 nm excitation, 525 nm emission, FL1) fluorescence. Relative median green fluorescence is then calculated for each microsphere and presented as a GlycoPrint.