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. 2023 Jan 11;13:567. doi: 10.1038/s41598-023-27634-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Binding of multiplex GlycoSense bead sets to biotinylated glycan standards conjugated to anti-biotin Fab labeled with DyLight 488. (A) SNA beads bind specifically to Siaα2,6Galβ1,4Glc. (B) MAL I beads bind specifically to Siaα2,3Galβ1,4GlcNAcβsp-Biotin. (C) GSL II beads bind specifically to GlcNAcβ1,4GlcNAc. (D) ECA-conjugated beads bind specifically to Galβ1,4GlcNAc. One analysis per concentration was performed for these assays. Values for sp-biotin Fab alone were subtracted from median FL1 values for each glycan Fab concentration, giving small negative values for some non-binding microspheres.