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. 2023 Jan 11;6:27. doi: 10.1038/s42003-023-04417-w

Fig. 2. Xerucitrinin A and 6-pentyl-α-pyrone produced by S. morookaensis strain Sm4-1986 display antifungal activity against Foc TR4.

Fig. 2

a SEM images showing the morphology of Foc TR4 spores treated by 3 mM xerucitrinin A for 72 h. b SEM images showing the morphology of Foc TR4 spores treated by 0.96 mM 6-pentyl-α-pyrone for 72 h. c Morphology of the normal Foc TR4 spores analyzed by SEM. d Transmission electron microscope (TEM) image of the Foc TR4 spores treated by 0.96 mM 6-pentyl-α-pyrone for 24 h. e Magnification of the squared area in (d) showing a massive cytoplasm without integral organelles. The double arrow indicates the thinned cell wall. f TEM image of the normal Foc TR4 spores. g Magnification of the squared area in (f) showing mitochondrion (indicated by arrows). The double arrow indicates the thickness of cell wall. h Response surface methodology analysis shows interaction effect of xerucitrinin A and 6-pentyl-α-pyrone on antifungal activity against Foc TR4. Scare bars in (a–d), and (f), 1 μm; and in (e) and (g), 500 nm.