Fig. 6. Rotation of the εNTD increases the distance to the εCTH1 binding site.

To highlight the relative rotation of the ε subunit between the half-up and down sub-states, the State 2 half-up ε subunit was superposed onto the State 2 down εNTD (shown in yellow). After the εNTD rotates about the γ subunit, the distance between εCTH1 and its binding site on γ is increased in the down sub-state and is less likely to attach to the central stalk.