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. 2023 Jan 11;8:3. doi: 10.1038/s41536-023-00276-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Ad-Cre deletion of PDGFRβ showed decrease in expression at (a) protein and (b) mRNA level. c BMP2 induced expression of Alp, Noggin, Id1 and Dlx5, while PDGF BB treatment blocked their upregulation in combined PPDGF BB + BMP2 treatment. Deletion of PDGFRβ using Ad-Cre partially restored Alp expression upon combined BMP2 + PDGF BB treatment. n = 3. Values are expressed as mean ± s.e.m. *p < 0.05, **p < 0.01, ***p < 0.001, # different from its vehicle treatment control. Statistical test: Two-way ANOVA with Tukey’s post hoc test.