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. 2023 Jan 11;13:562. doi: 10.1038/s41598-023-27660-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Computer aided design (CAD) models of the nest and cradle (Scale bars A–E = 1 mm; F = 300 µm). (A) Isometric view of nest; (B) Isometric view of cradle; (C) Top view of nest (a—outlet channel, b—back reservoir, c—inlet channel, arrows indicate direction of fluid flow); (D) Top view of cradle (d—nozzle, e—cell chamber which is open at the top to enable cell manipulation, f—injection channel, g—fin); (E) Top image of nest at 1 × magnification on Nikon SMZ18 stereomicroscope. The dimensions of the nest ‘chip’ are 8.8 mm × 8.2 mm × 3.6 mm (nest printed at 10 × in adaptive resolution in course mode above line h and fine mode below line h); (F) Top image of cradle at 8 × magnification with a Nikon SMZ18 stereomicroscope (cradles printed at 20 × in course infill mode).