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. 2023 Jan 11;13:562. doi: 10.1038/s41598-023-27660-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Microfluidic testbed for nest-cradle device (Scale bars = 1 mm). (A) Diagrammatic representation of testbed components (components and positions are not to scale); (B) Zoom in on model of three central cradles inserted into the nest, which acts as the roof with cell loading holes for each cradle; (C) Zoom in on model of the whole nest with ten cradles inserted; (D) Model of nest with tubing attached secured by titanium holder in 60 mm petri dish; (E) Photographic image of testbed set up in the laboratory with a Nikon SMZ18 stereomicroscope. An Olympus IX83 inverted microscope was used in place of the SMZ18 for particle tracking velocimetry.