Figure 1.

RhoA mutations induced morphological changes in MKN74 cells. (A) RhoA mRNA expression of MKN74 cells transduced with RhoA wild type or RhoA mutants, RhoA R5W, G17E, and Y42C, as determined by real-time PCR (N = 5). (B) RhoA protein expression in MKN74 cells transduced with a vector containing RhoA mutants by western blot analysis. GAPDH was used as an internal control. (C) Morphological changes in MKN74 cells transduced with a vector containing RhoA mutants (original magnification ×400, Scale bar = 50 µm). (D) Proliferation of these cells after 96 h of culture (N = 4). *P < 0.05.