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. 2023 Jan 9;22:15330338221146024. doi: 10.1177/15330338221146024

Figure 3.

Figure 3.

Introduction of RhoA mutations activated the ROCK signaling pathway, whereas inhibition of the ROCK signaling pathway unexpectedly enhanced cell motility. (A) Protein expression of the RhoA-ROCK signaling pathway in MKN74 cells transduced with a vector containing RhoA mutants. Transduction of the RhoA G17E and Y42C mutant-containing vector enhanced the expression of ROCK and phosphorylated cofilin proteins (N = 4). (B) The ROCK inhibitor, Y-27632, suppressed the RhoA G17E-induced phosphorylation of cofilin in a concentration-dependent manner (N = 4). (C) Migration and invasion assays of MKN74 cells transduced with RhoA WT and the RhoA G17E mutant using a transwell system with 10 μM Y-27632 (N = 4). Inhibition of the ROCK signaling pathway unexpectedly enhanced cell motility in both RhoA WT- and RhoA G17E-containing cells. (D) Invasion assays of MKN74 cells transduced with a RhoA WT- or RhoA G17E mutant-containing vector using a Matrigel-coated transwell system with 10 μM Y-27632 (N = 4). Cell invasion was promoted significantly in cells with RhoA WT but was not significantly changed in cells with the RhoA G17E mutant (N = 4). *P < 0.05.