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. 2023 Jan 11;14:4. doi: 10.1007/s12672-023-00611-8

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Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A mRNA level of S1PR2 detected by RT-qPCR after S1PR2 plasmid transfection of HCC cells; B protein level of S1PR2 detected by western blot after S1PR2 plasmid transfection of HCC cells; C statistical difference analysis related to the protein level of S1PR2 revealed by western blot; D activity of cells revealed by CCK8; E, F monoclonal assay for proliferation activity of cells; G and H: flow cytometry for cell cycle change; I, J flow cytometry for apoptosis; K statistical analysis of cell cycle change revealed by flow cytometry; L statistical analysis of apoptosis shown by flow cytometry *p < 0.05, **p < 0.01, ***p < 0.001, Vs shNC