Figure 9. Disease- and sex-specific differences in abundance of proteins related to cytoskeleton and muscle contraction.

(A) Clustered heatmap showing the condition’s mean abundance of proteins belonging to cytoskeleton- and muscle contraction–related GO terms. Annotation bars denote significant changes in condition (I) and in sex (II—effect in sex MR; III—effect in sex AS). Proteins described in the text are labeled. (B) Median log₂ of LFQ intensity of all proteins with actin binding (left) or myofibril (right) annotation. P-values in (B) are calculated using a Wilcoxon rank test. (C) Combined results (−log₁₀-transformed P-value) of GO term enrichment analysis in AS and MR versus CON shown in the direction of regulation they were found in. (D) Protein ratio of myosin heavy chains MYH6 and MYH7 in AS, MR, and CON. P-values in (D) are calculated using a Wilcoxon rank test. (E) Graphical illustration of regulated proteins belonging to the cortical cytoskeleton. The color indicates MR-specific increase (green). AS, aortic valve stenosis; CON, healthy control hearts; Cond., condition; MR, mitral valve regurgitation; ns, not significant.