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L. pneumophila infection induces little IL-12 p40/p70, although avirulent or killed L. pneumophila stimulates higher levels. TG-macrophages were infected with virulent L. pneumophila (Lp) or avirulent L. pneumophila (AvLp) as described in Materials and Methods or stimulated with formalin-killed L. pneumophila (kLp) or E. coli LPS. At 24 h, supernatants were collected and analyzed by ELISA for IL-12 p40/p70. Data are the means and SEM of three experiments.
