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. 2022 Jun 30;36(2):315–321. doi: 10.5713/ab.22.0059

Figure 2.

Figure 2

Dephosphorylation of PA FLA by sweet potato PAP in different temperatures. The phosphatase assay was performed in a 1-mL reaction mixture containing 4 μL of the enzyme (46.4 μg) and 1 μg of PA FLA substrate at pH 5.5 (Na-acetate) for 4 hours at 25°C, 37°C, and 55°C. Enzyme activity was defined as the amount of enzyme required to liberate 1 nmol of inorganic phosphate per min divided by mg of the protein. The data were expressed as the mean and standard errors from three experiments. PA FLA, Pseudomonas aeruginosa flagellin; PAP, purple acid phosphatase. a–c Means lacking common letters differ significantly (p<0.05).