Table 2.
Oligonucleotide primer sequences for RT-qPCR
| Gene | Sequence | Accession number |
|---|---|---|
| ACC | F: CACTTCGAGGCGAAAAAC R: GGAGCAAATCCATGACCA |
XM_015295697.2 |
| CAT | F: ACCAAGTACTGCAAGGCGAA R: TGAGGGTTCCTCTTCTGGCT |
NM_001031215.1 |
| EXFABP | F: GGAGGACCTTGCACATGA R: GTGTAGTTCCGCTCCCTA |
NM_205422.1 |
| FAS | F: CAATGGACTTCATGCCTC R: GCTGGGTACTGGAAGACA |
NM_205155.3 |
| GPx1 | F: AACCAATTCGGGCACCAG R: CCGTTCACCTCGCACTTCTC |
NM_001277853.2 |
| NOX1 | F: GCGAAGACGTGTTCCTGTAT R: GAACCTGTACCAGATGGACTTC |
NM_001101830.1 |
| NOX4 | F: CCTCTGTGCTTGTACTGTGTAG R: GACATTGGAGGGATGGCTTAT |
NM_001101829.1 |
| NRF2 | F: CAGAAGCTTTCCCGTTCATAGA R: GACATTGGAGGGATGGCTTAT |
NM_205117 |
| PPAR-γ | F: TCAGGTTTGGGCGAATGC R: CGCTCGCAGATCAGCAGA |
XM_040646063.1 |
| SOD | F: AGGGGGTCATCCACTTCC R: CCCATTTGTGTTGTCTCCAA |
NM_205064.1 |
| β-actin | F: ACCGGACTGTTACCAACA R: GACTGCTGCTGACACCTT |
NM_205518.1 |
| GAPDH | F: TTGGCATTGTGGAGGGTCTTA R: GTGGACGCTGGGATGATGTT |
NM_204305.1 |
RT-qPCR, real-time quantitative polymerase chain reaction; ACC, acetyl-CoA carboxylase; CAT, catalase; EXFABP, extracellular fatty acid-binding protein; FAS, fatty acid synthase; GPx1, glutathione peroxidase 1; NOX1, nicotinamide adenine dinucleotide phosphate oxidase 1; NOX4, nicotinamide adenine dinucleotide phosphate oxidase 4; NRF2, nuclear factor erythroid 2-related factor 2; PPAR-γ, peroxisome proliferator-activated receptor gamma; SOD, superoxide dismutase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.